Plasmid

Part:BBa_K1369010:Design

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pBAD LamA-L-SH3pep, pLamA-GFP, RR with direct recruitment (SH3 peptide). Ideal pBad concentrations w


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 3402
    Illegal SpeI site found at 19
    Illegal PstI site found at 4591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 13
    Illegal NheI site found at 1263
    Illegal NheI site found at 1811
    Illegal SpeI site found at 19
    Illegal PstI site found at 4591
    Illegal NotI site found at 5548
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2105
    Illegal EcoRI site found at 13
    Illegal BglII site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 3402
    Illegal SpeI site found at 19
    Illegal PstI site found at 4591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 13
    Illegal XbaI site found at 3402
    Illegal SpeI site found at 19
    Illegal PstI site found at 4591
    Illegal NgoMIV site found at 1789
    Illegal NgoMIV site found at 3857
    Illegal NgoMIV site found at 4140
    Illegal NgoMIV site found at 7822
    Illegal AgeI site found at 1047
    Illegal AgeI site found at 7982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3117
    Illegal BsaI site found at 3295
    Illegal BsaI site found at 5228
    Illegal BsaI.rc site found at 2953
    Illegal SapI site found at 1029
    Illegal SapI site found at 4080
    Illegal SapI site found at 4290


Design Notes

Scaffold protein


Source

Lactobacillus plantarum WCFS1

References